HCT 116-EGFP cell line

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HCT 116-EGFP Order

HCT 116-EGFP cell line
Catalog#
YC-C004-EGFP-P
Size
1*10^6
Transfection Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HCT 116-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
HCT 116-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Accession
CVCL_C8WS(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116
STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
EGFP validation
HCT 116-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the HCT 116 cell line, screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HCT 116-EGFP cell line that can maintain long-term, stable and high expression.
HCT 116-EGFP cell line
HCT 116-EGFP
Catalog#
YC-C004-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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